Submit Manuscript  

Article Details

The Novel Tank Test: Handling Stress and the Context Specific Psychopharmacology of Anxiety

[ Vol. 5 , Issue. 2 ]


Steven Tran and Robert T. Gerlai   Pages 169 - 179 ( 11 )


Background. The zebrafish has become a popular animal model for behavioral pharmacology. One of the greatest advantages of the zebrafish is its amenability for high throughput screens to identify anxiety altering compounds. The novel tank diving test is arguably one of the most commonly utilized behavioral tests for quantifying anxiety-like behavioral responses in zebrafish. However, a number of context-dependent anxiety-like behavioral responses have recently been reported in the literature for this task, and the validity of using a single measure, the diving response, has been questioned.

Objective. In this review, we discuss multiple behavioral parameters that can be used to evaluate performance in the novel tank diving test which can serve as indicators of anxiety. In addition, we also discuss the difficulties associated with interpretation of behavioral measures of anxiety, a question complicated by context- dependent behavioral responses induced by handling the fish with a net, a known stressor.

Conclusion. The zebrafish is a relative newcomer in the psychopharmacological analysis of anxiety, but the increasing number of efficient behavioural tests and the translational relevance of this species make the zebrafish a potentially useful laboratory species for screening of anxiolytic compounds.


Zebrafish, anxiety, novel tank test, psychopharmacology, anxiolytics, stress.


Department of Cell and Systems Biology, University of Toronto Mississauga, 3359 Mississauga Road North, DV 1022D, Mississauga, Ontario, L5L 1C6, Canada., Department of Psychology, University of Toronto Mississauga, 3359 Mississauga Road North, CC4004, Mississauga, Ontario, L5L 1C6, Canada.

Graphical Abstract:

Read Full-Text article